Q2 Laser Scanning Confocal Nanoscope 激光掃描共聚焦顯微鏡


具有單分子檢測功能的緊湊型定量細胞生物學儀器。

Q2是一種激光掃描納米顯微鏡(LSN),它結合了多種測量模式,適用於需要單分子檢測靈敏度的實驗定量生物學和材料科學應用。

The compact instrument for quantitative cell biology with single-molecule detection.

Q2 is a laser scanning nanoscope (LSN) that incorporates several measurement modalities for experimental quantitative biology and material sciences applications requiring the single molecule detection sensitivity.

 

 

 
Key Features of Q2 Laser Scanning Nanoscope:
  • Single- and multi-photon excitation on three separate input ports
  • Two channel acquisition
  • One, computer-controlled-aperture, pinhole
  • Fast scanning mirrors
  • Powered by VistaVision, a user-friendly software package for the acquisition of confocal images, FLIM/ FRET, RICS, and FFS (FCS, PCH, scanning FCS, N&B)

 

Excitation Modality
Three separate input ports allow for simultaneous alignment of one-photon excitation (laser diodes and supercontinuum laser) or multi-photon measurements (fiber laser, Ti:Sapphire laser).

 

Microscopes
Q2 can be interfaced with most commercial epifluorescence and upright microscopes.

 

Detectors
GaAs detectors, hybrid photomultiplier tubes, avalanche photodiodes.

 

Q2 Laser Scanning Confocal Nanoscope Catalog Download

特點

Microscope and Coupling
  • Olympus, Nikon, Zeiss and Leica
  • Inverted and upright
  • Left side port and back port
1p Excitation1
  • ISS laser launcher (models for 3, 4, and 6 laser diodes), wavelengths available from 375 nm to 640 nm; pulse interleave excitation (PIE)
  • Super-coninuum lasers, wavelength from 400 to 700 nm
2p Excitation1
  • Ultra fast femtosecond pulse Ti:Sapphire lasers
  • Ultra fast femtosecond pulse fiber lasers
Data Acquisition Unit
  • FastFLIM (Digital Frequency Domain FLIM)
    • Supports all above listed applications except particle tracking and nanoimaging
  • TCSPC card (Time Domain FLIM)
Detectors
  • GaAs PMT (Hamamatsu H7421 and H7422P models)
  • Hybrid PMTs (Hamamatsu R10467U models)
  • APDs
Positioning Controls2
  • ISS 3-axis control unit
  • ISS XY galvo scanning mirrors control unit
  • ISS Z-piezo control unit
  • Microscope built-in focusing control module
  • Automatic XY stages (ASI, Prior)
Software
  • VistaVision (Windows 7, 64-bit)
Computer & Monitor
  • 3GHz Processor, 8GB RAM, 200GB hard drive for 64-bit VistaVision
  • 27" 1280 x 1024 resolution monitor
  • Windows 7 64-bit Professional
Power Requirements
  • Universal power input: 110-240 V, 50/60 Hz, 400 VAC
  1. Q2 was fully evaluated and validated for using ISS laser launcher, Fianium SC-400 laser and Toptica FemtoFiber Pro 2p laser.
  2. VistaVision provides utilities for measurements in spectroscopy mode (at a single point), raster or orbit scan mode (2D XY), optical sectioning mode (3D), time-lapse mode, stage scan mode for multi wells, or a combination of them.

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